A Random Motility Assay Based on Image Correlation Spectroscopy

Scanning image correlation spectroscopy.

Molecular interactions are at the origin of life. How molecules get at different locations in the cell and how they locate their partners is a major and partially unresolved question in biology that is paramount to signaling. Spatio-temporal correlations of fluctuating fluorescently tagged molecules reveal how they move, interact, and bind in the different cellular compartments. Methods based o...

Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.

Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction, noise, and image morphology were examined analytically and their effects on I...

Cluster-Based Image Segmentation Using Fuzzy Markov Random Field

Image segmentation is an important task in image processing and computer vision which attract many researchers attention. There are a couple of information sets pixels in an image: statistical and structural information which refer to the feature value of pixel data and local correlation of pixel data, respectively. Markov random field (MRF) is a tool for modeling statistical and structural inf...

An ultrasensitive site-specific DNA recombination assay based on dual-color fluorescence cross-correlation spectroscopy

Site-specific exchange of genetic information is mediated by DNA recombinases, such as FLP or Cre, and has become a valuable tool in modern molecular biology. The so far low number of suitable recombinating enzymes has driven current research activities towards alteration of catalytic properties, such as thermostability or recognition sequences. However, identification and analysis of new mutan...

Investigating the motility of Ecoli using Fluorescence Correlation spectroscopy